Measures the level of vitamin D in your blood to check for deficiency.
Also known as: Vitamin D Test, Vitamin D 25-OH, 25-Hydroxy Vitamin D, Vitamin D3 Test
The Vitamin D Total (25-Hydroxy) Test measures the level of vitamin D in your blood and helps determine whether you have a deficiency, sufficient levels, or excess vitamin D. Vitamin D is essential for maintaining healthy bones, supporting muscle function, improving immunity, and helping the body absorb calcium effectively.
Although sunlight is the primary source of vitamin D, many people develop deficiency due to indoor lifestyles, limited sun exposure, dietary habits, aging, or certain medical conditions. A Vitamin D deficiency can lead to weak bones, muscle pain, fatigue, and an increased risk of osteoporosis.
The Vitamin D Total (25-Hydroxy) Test measures the concentration of 25-hydroxyvitamin D, the major circulating form of vitamin D in your bloodstream. This is considered the most reliable marker for evaluating your body's vitamin D status.
Doctors commonly recommend this test to diagnose vitamin D deficiency, monitor supplementation, investigate bone disorders, or assess unexplained muscle weakness and fatigue.
Benefits of vitamin D:
Common causes of vitamin D deficiency:
Best sources of vitamin D:
Daily recommended vitamin D intake:
Benefits of vitamin D testing:
A Vitamin D Test is recommended for:
You may benefit from this test if you have symptoms of vitamin D deficiency, such as:
1.Vitamin D Total (25-Hydroxy)
Measures 25-hydroxyvitamin D, the major circulating form of vitamin D in your bloodstream. It is considered the most reliable marker of your vitamin D status.
No fasting is required.
Before your test:
| Vitamin D level | Interpretation |
|---|---|
| Less than 20 ng/mL | Deficient |
| 20–30 ng/mL | Insufficient |
| 30–50 ng/mL | Sufficient |
| Above 100 ng/mL | High (requires medical evaluation) |
Reference ranges can differ between labs. Your report shows the range used by the lab.
No. Fasting is not required for a Vitamin D Test.
A small blood sample is collected from a vein.
Yes. OnHealth offers convenient home blood sample collection in the cities we serve.
Reports are generally available within 24–30 hours.
| Test name | Vitamin D Total (25-Hydroxy) Test |
|---|---|
| Location | Orai |
| Sample type | Blood |
| Number of parameters | 1 |
| Home sample collection | Available |
| Fasting | Not required |
| Report time | Within 24–30 hours |
| Recommended age | 5–99 years |
| Suitable for | Male & Female |
Individuals with deficiency, osteoporosis, or those taking vitamin D supplements should follow their doctor's recommendation. Others may include it in annual preventive health checkups.
